Hello Ma'am, Can you suggest me a method to clear chlorophyll...

Mahesh Kumar Panda
Mahesh Kumar Panda
Dec 13, 2021
protocol Protocol: Leaf Clearing Protocol to Observe Stomata and Other Cells on Leaf Surface
Hello Ma'am,
Can you suggest me a method to clear chlorophyll from flag leaf of rice?
I tried Lactic acid (85%) plus chloralhydrate based method but its not working for mature leave.

Thank You
Mahesh
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Nidhi Sharma Author Answered Dec 22, 2021

HHMI/Stanford University

Hi Mahesh,
I have not worked with rice leaves before. Did you try the 7:1 solution of 95% ethanol: acetic acid as mentioned in this protocol? You can try clearing for a longer time e.g., 2 days, and change the clearing solution twice a day in case the flag leaf is big and thick. Let me know if that works!
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