Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Tris-HCl pH 8, 1 M (autoclave) H2O Complete to 80 ml, adjust...
MX
Mande Xue Nov 21, 2021
Tris-HCl pH 8, 1 M (autoclave)
H2O Complete to 80 ml, adjust pH to 8 with NaOH, and then complete to 100 ml
above information is wrong, Tris-HCl pH 8 should adjust pH to 8 with HCl not NaOH
3
Views
Answer
Recommend
Follow
Share
1
answer
Sort by: most helpful / most recent
Juan S Ramirez-Prado Author Answered Nov 22, 2021
Centre of Microbial and Plant Genetics (CMPG),KU Leuven, Center for Plant Systems Biology, Leuven
Hello. This is not wrong because the pH of Tris-HCl by default is lower than 8. Hence, adding NaOH will allow the pH to raise to 8.
When you start with Tris base you have to add HCl to decrease the pH.
When you start with Tris base you have to add HCl to decrease the pH.
0 helpful
0 unhelpful
0 comments ShareMy answer
Write your answer...
References (optional)
Add more
Post an Answer