Hello, Thanks for sharing this protocol. I'm confused in Procedure...

protocol Protocol: Vesicle Isolation from Bacillus subtilis Biofilm
Hello, Thanks for sharing this protocol. I'm confused in Procedure part B-1. Before centrifugation(15,000 x g ), did the supernatant removed from plates by pipetting and then collected biofilm pellicle on the surface of the media by washing or using a cell scraper? Sincerely.
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