For Step # 18 - do you seal the coverslip while placing it on...

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Nikhil Raghuram
Jul 23, 2021
protocol Protocol: ImmunoFISH for Adherent Cultured Mammalian Cells
For Step # 18 - do you seal the coverslip while placing it on a heat block? If not, are there concerns regarding drying out the coverslip?
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Francesca Rossiello Author Answered Jul 27, 2021

The FIRC Institute for Molecular Oncology, IFOM, Italy

Hello,

No, I do not seal it. Even if the cover seems to dry out after the incubation, the staining works perfectly anyway.
I hope this helps, but do not hesitate to ask for more information.

Best,

Francesca
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