Keisuke Yoshida Author Answered Jul 9, 2021
Institute of Innovative Research, Tokyo Institute of Technology, Japan
For determining protein concentration in this experiment, I have used only BCA assay. I often use Bradford assay for recombinant proteins, , but it cannot be used in this experiment, because the detergent (SDS) is contained in the sample preparations. Other methods may also work, but I am not really sure about that.