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Hello, have you tried using different protein determination...

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Gustaf Degen Jul 5, 2021
protocol Protocol: Simple Method to Determine Protein Redox State in Arabidopsis thaliana
Hello, have you tried using different protein determination kits, e.g. DC protein assay. I just wonder if it is compatible with bromphenol blue? Cheers, Gustaf
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Keisuke Yoshida Author Answered Jul 9, 2021

Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Nagatsuta-cho 4259-R1-8, Midori-Ku, Japan

For determining protein concentration in this experiment, I have used only BCA assay. I often use Bradford assay for recombinant proteins, , but it cannot be used in this experiment, because the detergent (SDS) is contained in the sample preparations. Other methods may also work, but I am not really sure about that.
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