Hello there, I would like to ask, if I use the 0.4% trypan blue...

jl
jintao lang
Jul 1, 2021
protocol Protocol: Plant Tissue Trypan Blue Staining During Phytopathogen Infection
Hello there, I would like to ask, if I use the 0.4% trypan blue dye solution purchased directly, is it possible to dye directly? Does it need to be vacuumed? Thank you!
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Marta Berrocal-Lobo Author Answered Jul 5, 2021

Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Parque Científico y Tecnológico, UPM, Campus de Montegancedo, Spain

Dear Sr,
We prepare our own trypan blue staining solution which is described into this protocol, but you should check if the composition of your purchased solution is the same. In that case you might use similarly to us.
We never needed to make vacuum into leave plant tissues because the trypan blue solution, that we prepare, penetrates very fast into the leave dead tissues. In any case that will depends on the kind of tissue.
Let me know if you would need additional help.
Sincerely
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