Improve Research Reproducibility A Bio-protocol resource

Thanks for sharing this protocol. Have you ever done any staining...

Victoria Hewitt Jun 14, 2021

Protocol: Generation of Functional Mouse Hippocampal Neurons

Thanks for sharing this protocol. Have you ever done any staining to confirm you do not end up with any cortical neurons in your prep? If so could you share which markers you used?

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Francesco Tomassoni-Ardori Author Answered Jun 15, 2021

Neural Development Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick

Hi Victoria! The straight answer to you question is no, we have never tried any specific cortical marker on those cultures. The hippocampus has a very well defined structure which allows a clean dissection (as you can see in Fig.6). Being the "starting material" so pure, you would expect a minimal or negligible cortical contamination, if any. All the best!

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