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HiI need to isolate pbmc for t-cells (CD4,CD8) study so can...
Hi
I need to isolate pbmc for t-cells (CD4,CD8) study so can i use EDTA tube for separation of cells without hampering the cell viability? Which anticoagulant will be best as i need to freeze the pbmcs at -80 degree celsius and transport it to the lab for the further study.
I need to isolate pbmc for t-cells (CD4,CD8) study so can i use EDTA tube for separation of cells without hampering the cell viability? Which anticoagulant will be best as i need to freeze the pbmcs at -80 degree celsius and transport it to the lab for the further study.
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Santosh K Panda Author Answered Jun 2, 2021
Infectious Disease Biology, Institute of Life Sciences, India
Yes, You could use EDTA. But I would prefer heparin for PBMC isolation.
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