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Can we subtitute RbCl with Magnesium Chloride? If yes, then...

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Munib Ahmad May 29, 2021
protocol Protocol: A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
Can we subtitute RbCl with Magnesium Chloride? If yes, then what should be kept its concentration?
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Diego Wengier Author Answered Aug 3, 2021

Hi Munib,

I've replaced RbCl with Potassium Chloride in the past and the protocol still holds, however, i was never able to get efficiencies at the same level as with RbCl. I hope this helps!
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