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Hello there, I am wondering if the number of leaf disc used...
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TINA R AGARWAL May 20, 2021
Hello there, I am wondering if the number of leaf disc used has any significance in electrolyte measurement. I am working with cucumber leaves and am using 4 leaf disc per plant in 10 ml DI-water, as this volume helps me to measure the conductivity (we have big electrode like meter (looks Read more 
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Fumiaki Katagiri Author Answered May 21, 2021
Dept of Plant and Microbial Biology, University of Minnestoat, USA, USA,
Dear Tina,
The number of discs, the volume of the well, and such are by no means appropriate in different cases. If your conductivity meter requires a large volume, you would need to put a higher amount of tissue - the sensitivity of the measurement depends on tissue amount/water volume. On the other hand, if you use a larger amount of tissue, the response of a higher number of cells get averaged, which may result in an underestimate of SD in the cell response. You just need to decide the scale of measurement considering the practicality and the potential trade-offs in different experimental system.
The number of discs, the volume of the well, and such are by no means appropriate in different cases. If your conductivity meter requires a large volume, you would need to put a higher amount of tissue - the sensitivity of the measurement depends on tissue amount/water volume. On the other hand, if you use a larger amount of tissue, the response of a higher number of cells get averaged, which may result in an underestimate of SD in the cell response. You just need to decide the scale of measurement considering the practicality and the potential trade-offs in different experimental system.
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