Has anyone tried this method with leaf tissue, can the whole...

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TINA R AGARWAL
May 18, 2021
protocol Protocol: Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress Through Schiff’s Reagent: A Histochemical Approach
Has anyone tried this method with leaf tissue, can the whole leaf be stained or do we need to make section or leaf disc is needed for staining any suggestion?
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Bedabrata Saha Author Answered May 19, 2021

Newe Ya'ar Reseach Centre, Agricultural Research Organization, Ramat Yishay Ramat Yishay

Hi Tina,
Thank you for asking. We ourselves have used this protocol for rice leaf tissue segments.
Sahoo, S., Saha, B., Awasthi, J. P., Omisun, T., Borgohain, P., Hussain, S., ... & Panda, S. K. (2019). Physiological introspection into differential drought tolerance in rice cultivars of North East India. Acta Physiologiae Plantarum, 41(4), 1-15.
Besides whole leaf can also be used for staining. It just depends on how you want to represent. If you are facing any problem while performing the experiment feel free to contact us again.
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Jay Prakash Awasthi Author Answered May 19, 2021

Govt. college lamta, balaghat Balaghat

We can take whole leaf or small leaf section for this experiment. Thanks for your questions.
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