Dear Dr. Gillotin, I tried this protocol and I got a pretty...

protocol Protocol: Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
Dear Dr. Gillotin, I tried this protocol and I got a pretty good separation of nuclear and chromatin fraction. I have two questions

1. Is it correct to assume that the chromatin fraction is more the insoluble nuclear fraction, meaning that it also contains insoluble nuclear spe
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Sébastien Gillotin Author Answered Jun 23, 2021

Discovery Research, Neuroscience, MSD

Hello,
To answer your first point, I would say yes. The fractionation is never completely pure and should be confirmed by other experiments
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