what is M3 (sm+)? and what stands for sm+ ?

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Pashma Nawaz
May 3, 2021
protocol Protocol: Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders
what is M3 (sm+)? and what stands for sm+ ?
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Annika Zink Author Answered May 10, 2021

Heinrich Heine University Düsseldorf

Dear Pashma,

hadia mujahid is right. M3, also called sm+, is the final culturing media for the NPCs. Once the generation of the NPCs is completed, cells are kept in sm+ throughout the culture. You can find the media composition in Tab. 1 (basic media) and Tab. 2.
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hadia mujahid Answered May 8, 2021

hadia mujahid

this is a media used for culturing the neural cells. it could be used for freezing, moreover you can look for the functions through the composition provided in this article.
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