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Hello. I cannot get this protocol to work, I was wondering if...

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Alex Wardale Apr 27, 2021
protocol Protocol: Dot Blot Analysis of N6-methyladenosine RNA Modification Levels
Hello. I cannot get this protocol to work, I was wondering if you have a reference troubleshooting guide? I use the same primary and secondary antibodies, purified and serial diluted mRNA, and same buffers, the only difference being I use BSA instead of non-fat milk.
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Hao Yu Author Answered May 9, 2021

Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore

There are many factors determining whether you can get good signals of dot blot, such as mRNA qualities, m6A abundance in the samples, crosslinking efficiency, etc. We suggest you to include some positive controls and try again.
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Alex Wardale Answered Apr 29, 2021

Nord University

This issue has been resolved.
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