What do you think about the addition of mitomycin c after the...

Prasanth Manohar
Prasanth Manohar
Apr 23, 2021
protocol Protocol: Screening for Lysogen Activity in Therapeutically Relevant Bacteriophages
What do you think about the addition of mitomycin c after the patch-plate assay for phage induction?
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Jeremy J Barr Author Answered Apr 27, 2021

Monash University Clayton

Hi Prasanth!
It is an interesting idea with addition of Mitomycin C for the patch plate. We've typically seen quite strong auto-induction for lysogens in this assay and haven't needed mitomycin, but it may increase sensitivity of the assay
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