Improve Research Reproducibility A Bio-protocol resource

8.YPRAA medium (1 L) 10 g yeast extract 20 g peptone 20 g...

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song hong Apr 11, 2021
protocol Protocol: Functional Evaluation of the Signal Peptides of Secreted Proteins
8.YPRAA medium (1 L) 10 g yeast extract 20 g peptone 20 g raffinose 20 g Agar A Add distilled water to 1 L, autoclave for 15 min at 121 °C Add antimycin A to a final concentration of 2 mg/ml just before pouring medium plates， final concentration of antimycin A should be 2 μg/ml ???? 10. Read more Read less more less
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Weixiao Yin Author Answered Apr 12, 2021

Huazhong Agricultural University

Thank you to find out the imperfection. 1. The final concentration of antimycin A should be 2 μg/ml in our experiments. 2. The missing issue is indeed Na2HPO4.
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