Hi, Very helpful protocol Just a short question: Is the 1/2...

Muahammad Ahmad
Muahammad Ahmad
Mar 16, 2021
protocol Protocol: Quantification of Root Colonizing Bacteria
Hi,
Very helpful protocol
Just a short question: Is the 1/2 MS medium with sugar or without sugar? Thank you
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Axel de Zelicourt Author Answered Mar 17, 2021

Institut of Plant Sciences Paris-Saclay (IPS2), , UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, , France, France,

hello Muhammad

we used the MS medium basal salt and nothing else (no addition of vitamins or hormones) and of absolutely no sugar at all, as the bacteria would develop extremely rapidly and cover your plant :)
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