Should you plate the same number of cells for all treatment...

protocol Protocol: Clonogenic Assay
Should you plate the same number of cells for all treatment concentrations and PE controls. What is the general amount plated for an immortalized cell line being treated with chemo-drug in a 6 well plate?
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Sharavan Ramachandran Answered Mar 9, 2021

Sharavan Ramachandran

Hello Sam,

You should seed the same number of cells across all the groups. You can seed 500 cells per well in a 6 well plate. For more information, please refer to my answer to Khem's question.
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