Hello,Thanks for the protocol. You mention that these SN can...

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Preeti Kute
Feb 12, 2021
protocol Protocol: Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex
Hello,
Thanks for the protocol. You mention that these SN can be stored at -80 for a year. What is the exact application of these SNs? Have you also done an EM to check the integrity of these synaptoneurosomes? Also, people use synaptoneurosomes for synaptic stimulation, have you trie
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Ariel Diaz Author Answered Feb 19, 2021

Yerkes National Primate Research Center Atlanta

Hi Preeti,

Thank you for reading our protocol. These SNs can be used in all of the applications you mentioned; however, if you store them at -80, you will compromise the SNs integrity. If you want to perform functional and structural studies, you should use fresh SNs. Additionally, the EGTA in the buffers can be decreased or removed to reduce the interference with calcium signaling (the protease activity will increase too).

Best,

Ariel
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