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HelloI think I did not ask my questions well. The pellet from...

as
ade saf Jan 15, 2021
protocol Protocol: Isolation, Purification, and Characterization of Ginger-derived Nanoparticles (GDNPs) from Ginger, Rhizome of Zingiber officinale
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I think I did not ask my questions well. The pellet from the ultracentrifugation step (isolation step) was dissolved in PBS (as shown). Should this solution be clear? Or should this concentration exist? And is the purification step necessary? Or can the same solution be used for img Read more Read less more less
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kun chen Answered Sep 16, 2021

I want to know if you have obtained bands in sucrose density gradient centrifugation?
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Chunhua Yang Author Answered Jan 17, 2021

Institute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Digestive Disease Research Group, Georgia State University, Atlanta, USA

This solution is looking good. If you used sterile PBS, then it can be used in in vitro experiments.
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Junsik Sung Author Answered Jan 17, 2021

Georgia state university Atlanta

Dissolved pellets in PBS shouldn't be looking clear. Purification process is an important step for later use.
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