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HelloAt the end of isolation step, after using the cell strainer,...

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ade saf Jan 14, 2021
protocol Protocol: Isolation, Purification, and Characterization of Ginger-derived Nanoparticles (GDNPs) from Ginger, Rhizome of Zingiber officinale
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At the end of isolation step, after using the cell strainer, the resulting liquid contains particles that settle and form two phases. Should we have a completely clear liquid, or is the presence of this ghost-like particle natural and represent the exosomes?
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Chunhua Yang Author Answered Jan 15, 2021

Institute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Digestive Disease Research Group, Georgia State University, Atlanta, USA

The settled particles are normally large particles (>1 um) and exosomes are in the clear liquid. You can only get the nano-sized exosomes by ultracentrifugation.
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