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Dear ProfI am looking for a protocol to be used for homogenization...

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Niti Sharma Jan 11, 2021
protocol Protocol: Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords
Dear Prof

I am looking for a protocol to be used for homogenization of animal tissues for estimation of antioxidant parameters (SOD,Catalase, Glutathione peroxidase, GSH, MDA etc).
I have been using 50mM phosphate buffer (7.4) containing 0.1 M EDTA for extraction. Is there Read more Read less more less
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Oliver Wirths Author Answered Jan 13, 2021

Molecular Psychiatry, Dept. of Psychiatry and Psychotherapy, University Medical Center Göttingen (UMG), Germany

Dear Niti Sharma,
I apologize but we have no data on whether the indicated protocol works for the estimation of antioxidant enzymes as this is something we have never tested.

Kind regards,
Oliver Wirths
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