Hi, I'm wondering about the recipe for elution buffer. Why there...

Protocol: Purification of His-ubiquitin Proteins from Mammalian Cells

Hi, I'm wondering about the recipe for elution buffer. Why there are so many concentrations of Tris, 2-ME, SDS? In my knowledge, too many concentrations of the amine-based buffer can reduce the nickel ion, too many concentrations of 2-ME cause a reduction of nickel ion, and too many concen Read more Read less

Answer

Recommend

Follow

Share