When preparing the Fix solution, it is stated that AlexaFluor...

克东 翚
Oct 30, 2020
protocol Protocol: Double Labeling of Microtubules and Actin Filaments in Maize Leaf Division Zone
When preparing the Fix solution, it is stated that AlexaFluor 568-Phalloidin needs to be added, and Materials and Reagents only need to have Goat anti-mouse AlexaFluor 568 and AlexaFluor 488-Phalloidin, so here is the mistake of writing AlexaFluor 488-Phalloidin as AlexaFluor 568-Phalloidi Read more Read less more less
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Michelle Facette Author Answered Nov 4, 2020

Biology, University of Massachusetts, Amherst, United States

Yes - thank you for catching this and our apologies. The fluor on the Phalloidin should be different than the fluor on the antibody. Thus, it should state AlexaFluor 488-Phalloidin is added (if using Goat anti-mouse AlexaFluor 568). These two colors can be reversed if you wish - we find the brighter actin staining easier to see - but either way it will work, as long as the microtubules and actin filaments are labelled with different colors.
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