When preparing the Fix solution, it is stated that AlexaFluor 568-Phalloidin needs to be added, and Materials and Reagents only need to have Goat anti-mouse AlexaFluor 568 and AlexaFluor 488-Phalloidin, so here is the mistake of writing AlexaFluor 488-Phalloidin as AlexaFluor 568-PhalloidiRead moreRead less
Biology, University of Massachusetts, Amherst, United States
Yes - thank you for catching this and our apologies. The fluor on the Phalloidin should be different than the fluor on the antibody. Thus, it should state AlexaFluor 488-Phalloidin is added (if using Goat anti-mouse AlexaFluor 568). These two colors can be reversed if you wish - we find the brighter actin staining easier to see - but either way it will work, as long as the microtubules and actin filaments are labelled with different colors.