Dear authors, Thank you for this excellent protocol. This is...

protocol Protocol: CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
Dear authors,

Thank you for this excellent protocol. This is very helpful.
Just a note: can you please check the recipe for pyrimethamine? Under 3b the 1:1000 dilution mentioned would result in a 10 uM solution, rather than a 100 uM.
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Franziska Mohring Author Answered Oct 21, 2020

London School of Hygiene and Tropical Medicine, UK

Thanks for spotting this. It should be 50 ul of the Pyrimethamine aliquot to 5 ml RPMI, instead of 50 ml.
The final concentration in the culture is 100 nM.
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