Thank you for this excellent protocol. This is very helpful. Just a note: can you please check the recipe for pyrimethamine? Under 3b the 1:1000 dilution mentioned would result in a 10 uM solution, rather than a 100 uM.
London School of Hygiene and Tropical Medicine, UK
Thanks for spotting this. It should be 50 ul of the Pyrimethamine aliquot to 5 ml RPMI, instead of 50 ml. The final concentration in the culture is 100 nM.