Dear,Thank you for this protocol. I have a small question. If...

protocol Protocol: Cell Fractionation of Pseudomonas aeruginosa
Dear,

Thank you for this protocol. I have a small question. If a French Press is not available, can this step be replaced by an alternative (e.g. sonication) step?

Best regards,
Marie
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Kim R Hardie Author Answered Dec 25, 2021

School of Life Sciences, University of Nottingham, United Kingdom

Dear Marie,
It depends what your final sample will be used for. The French Press lyses in a different way to sonication, and is better for bigger volumes as the sample will not warm up. It is worth trying another lysis if the French press is not available to see if it works for you, Regards, Kim
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