Hi, I am a student and I try to understand a research article...

protocol Protocol: Flow Cytometric Detection of Reactive Oxygen Species
Hi, I am a student and I try to understand a research article that about treatment of antibiotic-resistant bacteria by encapsulation of ZnO nanoparticles. This article use the ros activity to inhibit the bacteria and they analyze ros by DCF-DA.
I wondering why they have to pretreated
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