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Hi, were you able to see over expression of your his-tagged...

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Thaneswary Rajanderan Aug 29, 2020
protocol Protocol: Purification of His-ubiquitin Proteins from Mammalian Cells
Hi, were you able to see over expression of your his-tagged proteins using silver staining?
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Vanesa Olivares-Illana Author Answered Aug 31, 2020

Instituto de Física, Universidad Autónoma de San Luis Potosí

Hello
I tried a couple of times, and I could see the overexpression but when the proteins are small, but if your protein is big, it will be more difficult. I hope this could help you.
Vanesa
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