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Dear Kate, You can just order the sequences as reported by the...

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A. Marieke Oudelaar Jul 27, 2020
protocol Protocol: Low-input Capture-C: A Chromosome Conformation Capture Assay to Analyze Chromatin Architecture in Small Numbers of Cells
Dear Kate,

You can just order the sequences as reported by the Capsequm software.

An example:

For the Hba-1 promoter (DpnII fragment coordinates (mm9):chr11:32182969-32183821), we use the following oligos:

GATCCATGGTAGCACAGGGCAGCTAAGATGCAAGTCTG Read more Read less more less
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Kate Liddiard Answered Jul 28, 2020

Cardiff University

Thanks so much for clarifying. I just had a moment where I started to doubt my understanding of the approach! I will order oligos in the same orientation as they come from the Capsequm design software.
Very best wishes and thanks for all your guidance.
Kate
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