I'd like to confirm concentration of indomethacin and insulin in 'Adipocyte differentiation medium'. Is it final concentration of these components given in parentheses in 'Recipes' No. 8 ?
Oral & Maxillofacial Surgery, School of Dentistry of Ribeirao Preto - University of Sao Paulo, 2019-
Dear colleagues,
This is the correct recipe:
Adipocyte differentiation medium 180 ml of D-MEM 20 ml of fetal calf serum (final concentration = 10%) 2 ml of gentamycin (final concentration = 50 μg / ml) 250 μl of amphotericin B (final concentration = 0.3 μg / ml) 2 ml of dexamethasone (final concentration = 10E-6 M ) 2 ml of 3-isobutyl-1-methylxanthine (final concentration = 0.5 mM) 260 μl of indomethacin (final concentration = 60 µM) 150 μl of insulin (final concentration = 10 µg / ml)
We apologize for the mistake and the inconvenience. Best regards.