I'd like to confirm concentration of indomethacin and insulin...

YK
Yuko Kaku
Jul 8, 2020
protocol Protocol: Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation
I'd like to confirm concentration of indomethacin and insulin in 'Adipocyte differentiation medium'.
Is it final concentration of these components given in parentheses in 'Recipes' No. 8 ?
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
2 answers
Sort by: most helpful / most recent

Yuko Kaku Answered Jul 14, 2020

Nippi Research Institute of Biomatrix

Dear Dr. Freitas,

Thank you for taking the time to reply.
As it's first time for me to isolate and differentiate MSC, your article was very helpful.

Best Regards.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Gileade P. Freitas Author Answered Jul 10, 2020

Oral & Maxillofacial Surgery, School of Dentistry of Ribeirao Preto - University of Sao Paulo, 2019-

Dear colleagues,

This is the correct recipe:

Adipocyte differentiation medium
180 ml of D-MEM
20 ml of fetal calf serum (final concentration = 10%)
2 ml of gentamycin (final concentration = 50 μg / ml)
250 μl of amphotericin B (final concentration = 0.3 μg / ml)
2 ml of dexamethasone (final concentration = 10E-6 M )
2 ml of 3-isobutyl-1-methylxanthine (final concentration = 0.5 mM)
260 μl of indomethacin (final concentration = 60 µM)
150 μl of insulin (final concentration = 10 µg / ml)

We apologize for the mistake and the inconvenience.
Best regards.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer