Hi, I'd like to ask some questions about reverse transcription....

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May 24, 2020
protocol Protocol: mRNA Stability Assay Using Transcription Inhibition by Actinomycin D in Mouse Pluripotent Stem Cells
Hi, I'd like to ask some questions about reverse transcription. In this protocol and other articles I have read, the same quality of total RNA was used at different time points for reverse transcription.If my target mRNA decays at the same rate as total RNA, am I not getting its half-life? Read more Read less more less
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