Why we can't extract cytoskeleton from a cell using...

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Annoymous :)
May 6, 2020
protocol Protocol: Binding Assay of Cytosolic Proteins to the Cytoskeleton
Why we can't extract cytoskeleton from a cell using centrifugation/sedimentation?
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Annoymous :) Answered May 7, 2020

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Thanks a lot for answering 😉
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Giampiero Cai Author Answered May 7, 2020

Dipartimento Scienze della Vita, University of Siena, Italy

Dear colleague, in principle it would be possible but it is practically very difficult to identify the parameters of centrifugation or sedimentation because the cytoskeleton is not a homogeneous compartment, the cytoskeletal elements can have different length, they can form aggregates, therefore having a different mass. it is therefore technically complicated.
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