Hello, when OMVs were filtered through a bottle top filter of...

protocol Protocol: Preparation of Outer Membrane Vesicle from Escherichia coli
Hello, when OMVs were filtered through a bottle top filter of pore size 0.45 μm, how to keep the bottle top filter sterile? (Is the device a disposable?). Thank you.
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Won Hee Lee Author Answered May 6, 2020

Life Sciences Department, Pohang University of Science and Technology (POSTECH), Republic of Korea

For preparation OMVs, I used disposable bottle top filter [Corning® bottle-top vacuum filters, cellulose acetate membrane, sterile, pore size 0.45 μm, membrane diam. 70 mm, funnel capacity 500 mL, case of 12, CLS430514-12EA]
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer