Hello, when OMVs were filtered through a bottle top filter of pore size 0.45 μm, how to keep the bottle top filter sterile? (Is the device a disposable?). Thank you.
Life Sciences Department, Pohang University of Science and Technology (POSTECH), Republic of Korea
For preparation OMVs, I used disposable bottle top filter [Corning® bottle-top vacuum filters, cellulose acetate membrane, sterile, pore size 0.45 μm, membrane diam. 70 mm, funnel capacity 500 mL, case of 12, CLS430514-12EA]