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Hi,Thank you for the protocol.Sorry this question comes a long...

JD
Joshua Dubowsky May 5, 2020
protocol Protocol: Dimethylmethylene Blue Assay (DMMB)
Hi,

Thank you for the protocol.

Sorry this question comes a long time from submission but you have never answered the question about the TRIS. How much TRIS to add to the DMBB per litre?
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Joshua Dubowsky Answered May 6, 2020

Joshua Dubowsky

I have solved the issue for people who cannot get this assay to work. Simply add 5mL 100% ethanol per litre to the mix OR, within 15 minutes of the assay, add 1 part 2M TRIS per 10 parts DMMB reagent. This is what the TRIS was for.
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