Hi, Brian:is it important to use the French press before vacuum...

Hi Yongfang,

We chose this particular French press because:

1) Its lid and plunger allow us to keep the rosettes submerged in the buffer during the vacuum infiltration,
2) it fits inside our vacuum chamber,
3) it is the perfect diameter to hold 6-week old Arabidopsis rosettes and
4) and it is easy to clean.

Any container fulfilling these requirements could be used in place of the French press we recommend.

Best of luck,
Brian