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First of all thank you for sharing this protocol with the scientific...

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Ana Velloso Feb 14, 2020
protocol Protocol: Dimethylmethylene Blue Assay (DMMB)
First of all thank you for sharing this protocol with the scientific community. I would like to perform this in synovial fluid, which I know I have to treat first with hyaluronidase otherwise will not work. I was wondering if you have any other suggestions or protocols when we use synovia Read more Read less more less
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Lance Corey Answered Feb 16, 2020

Advanced Botanical Consulting and Testing

There are 2 problems with your desire to use this protocol with synovial fluid. First, note 4 indicates it does not with with oligosaccharides shorter than 4 sugars. Hyaluronidase cleaves the polysaccharide into disaccharides. Second, note 5 indicates it doesn't work with non-sulfinated GAGs, such as hyaluronic acid. Looking at the composition of synovial fluid, it seems to only contain hyaluronic acid.

Lance
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