Hi, I am an undergraduate student studying CRISPR. Why is it...

RS
Rachel Spencer
Feb 6, 2020
protocol Protocol: Generation of Targeted Knockout Mutants in Arabidopsis thaliana Using CRISPR/Cas9
Hi, I am an undergraduate student studying CRISPR. Why is it necessary to use two plasmids, one to put the sgRNA sequence into, and then to put that sgRNA cassette into the second? Why cant the sgRNA cassette just be made directly into the second plasmid? Thank you for any explanation for Read more Read less more less
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