I am curious to know what you think would happen if, instead...

protocol Protocol: In vitro Culture of Human PBMCs
I am curious to know what you think would happen if, instead of isolating the PBMCs first, and then culturing them in medium, one simply diluted fresh blood (anti coagulated using either EDTA or citrate) into medium 1:1. That would not only be faster, avoiding the somewhat laborious ficoll Read more Read less more less
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Santosh K Panda Author Answered Feb 4, 2020

Infectious Disease Biology, Institute of Life Sciences, India

The contents released from a little lysis of RBCs interfere with the monocyte functions.
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