Hi,I am isolating PBMC from undiluted human blood using histopaque...

VV
Vikas Verma
Jan 23, 2020
protocol Protocol: Isolation of Human PBMCs
Hi,
I am isolating PBMC from undiluted human blood using histopaque 1119 and 1077(Sigma). In our system, we can not dilute the blood because simultaneously we need plasma in original concentration. As a result we are unable to get the clear separation at the 1077-1119 interface. the l
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Santosh K Panda Author Answered Jan 31, 2020

Infectious Disease Biology, Institute of Life Sciences, India

If you need the plasma, 1st separate the plasma, then dilute the remaining part of the blood 1:2 and layer it on histopaque.
It will work.
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