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How long are isolated mitochondria active at room temperature?...

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Ricky Vo Jan 17, 2020
protocol Protocol: Isolation of Mitochondria from Potato Tubers
How long are isolated mitochondria active at room temperature? (Minutes/hours?) Thank you.
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Ian Max Møller Author Answered Jan 22, 2020

Department of Molecular Biology and Genetics, Science and Technology, Aarhus University, Denmark

Potato tuber mitochondria purified by a similar method could be stored for at least 3 h at 25oC or 48 h on ice without a decrease in their respiration rate provided they were supplied with coenzymes (NAD+, TPP, CoA), which they lost during the incubation.

Neuburger, M. and Douce, R. 1983. Biochem. J. 216, 443-450
Neuburger, M. et al. 1984. Arch. Biochem. Biophys. 229, 250-258
Neuburger, M. et al. 1985. Biochem. J. 78, 405-410
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