Dear Dr. Gillotin,Do I need to make E1, E2 and E3 buffer fresh...

Arvind Panday
Arvind Panday
Jan 10, 2020
protocol Protocol: Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
Dear Dr. Gillotin,
Do I need to make E1, E2 and E3 buffer fresh all the time? If yes for how long they would be good at 4 degree or on ice? My 2nd question is could I use benzonase for western blot purpose? or is it recommended to use benzonase for pull down and sonication for western
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