I tried to do the second Golden Gate Reaction in silico before...

protocol Protocol: Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9
I tried to do the second Golden Gate Reaction in silico before doing the experiment, and it did not work. Is it possible that the vector needed is pYTK095 instead of pYTK083?
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Aashiq H Kachroo Author Answered Dec 24, 2019

Concordia University

Hi Aaron

Thanks for pointing the error. You are right, the second round Golden Gate cloning uses pYTK095 plasmid. We will correct the error soon.
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