Why don't you directly add the bromocresol purple to the 0.5...

WT
Warner Talsma
Dec 17, 2019
protocol Protocol: Evaluation of Root pH Change Through Gel Containing pH-sensitive Indicator Bromocresol Purple
Why don't you directly add the bromocresol purple to the 0.5 MS medium instead of transferring the seedlings to a new plate?

Kind Regards,
Warner Talsma
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Aparecida L. Silva Author Answered Dec 20, 2019

Ciências Biológicas, ESALQ/USP, Brasil, Brasil,

Warner I do not put direct bromocresol blue in MS medium because of the sensitivity of the medium with blue bromocresol reagent in pH changes, also because to visualize the extracellular medium alkalinization caused by the RALF1 peptide we need to concentrate the roots on a hard surface, to facilitate visualization of alkalinization.


Sincerely
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