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What do you recommend for fixing the muscle tissue when using...

JW
Joseph Walters Dec 3, 2019
protocol Protocol: Muscle Histology Characterization Using H&E Staining and Muscle Fiber Type Classification Using Immunofluorescence Staining
What do you recommend for fixing the muscle tissue when using Dystrophin antibody?
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Shihuan Kuang Author Answered Dec 5, 2019

Department of Animal Sciences, Department of Health & Kinesiology, Center for Cancer Research, Purdue University

We usually stain dystrophin on muscle cross-section just using typically fixation with 4% PFA for 10min. The staining is always good. For whole muscle tissue, it can be fixed for 2-4 hours dependent on the muscle size, and processed with glycine for 2hours, PBS wash 0.5h following dehydrate with 15% and 30% sucrose overnight. The staining is also good no matter is in embryo or adult muscle tissue.
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