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Variya Nemidkanam Nov 10, 2019
Thank you for the helpful protocol.
I already follow your protocol but I facing the sterility problem.
How do you dealing with bacterial contamination in the isolated vesicle?
Best regards,
I already follow your protocol but I facing the sterility problem.
How do you dealing with bacterial contamination in the isolated vesicle?
Best regards,
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Junsik Sung Author Answered Nov 11, 2019
Georgia state university Atlanta
Hi Variya,
In order to avoid contamination, all the agents should be sterilized and filtrate with 0.22 um filter. After suspension, it needs to be sterilized under UV light for about 30 min before you use it for in vitro and in vivo experiment. I hope this helps for your work. Please let me know if you have any other questions on the protocol. Thank you.
In order to avoid contamination, all the agents should be sterilized and filtrate with 0.22 um filter. After suspension, it needs to be sterilized under UV light for about 30 min before you use it for in vitro and in vivo experiment. I hope this helps for your work. Please let me know if you have any other questions on the protocol. Thank you.
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