Hello! I still have another question,GSSG is hydrolysis to two...

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LIAO liping
Oct 22, 2019
protocol Protocol: Fluorometric Estimation of Glutathione in Cultured Microglial Cell Lysate
Hello! I still have another question,GSSG is hydrolysis to two molar GSH ,wich means at the standard curve the same concentration of GSSG and GSH, GSSG will have almost two times fluorecence signal than GSH ,but as standard curve of yours and mine shows that it is not? How to explain thos Read more Read less more less
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Vikas Singh Author Answered Oct 23, 2019

Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, India

Hi, I think it is really difficult to get the fluorescence in such a way that GSSG standard show double the fluorescence of GSH standard. This may be due to the rapid oxidation of GSH once it comes in solution form due to oxidizing environment which might also play a role in converting GSH into GSSG.
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