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The AP buffer needs to be added NaCl and Glucose to ensure the...

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Zhang Liming Oct 17, 2019
protocol Protocol: Quantitation of Regulatory Activity for the Complement Alternative Pathway Using an Adaptation of the AP50 in vitro Assay
The AP buffer needs to be added NaCl and Glucose to ensure the osmotic pressure.
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Jillian Maree Carr Author Answered Oct 23, 2019

College of Medicine and Public Health, Flinders University, Australia

correction - NaCl but no glucose. Tablets are from oxoid for complement fixation test http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=BR0016&cat=&sec=1&c=UK&lang=EN
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Jillian Maree Carr Author Answered Oct 23, 2019

College of Medicine and Public Health, Flinders University, Australia

Hi Zhang
You are correct, but in our protocol the veronal buffer is made up from a barbitone tablet that already contains glucose and NaCl
Jill
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