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Why the absorbance of the blank was higher than all of my samples...

ZL
Zhang Liming Oct 17, 2019
protocol Protocol: Quantitation of Regulatory Activity for the Complement Alternative Pathway Using an Adaptation of the AP50 in vitro Assay
Why the absorbance of the blank was higher than all of my samples when I tried this protocol
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Jillian Maree Carr Author Answered Oct 23, 2019

College of Medicine and Public Health, Flinders University, Australia

Hi Zhang
not sure - your blank should just be the AP buffer, check there is not a pH indicator or that the gelatin isn't really coloured?
Jill
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