Whether we could scale up the assy for 3ml UV Vis spectrophotometry...

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Akhil M
Oct 11, 2019
protocol Protocol: Trehalase Activity in Arabidopsis thaliana Optimized for 96-well Plates
Whether we could scale up the assy for 3ml UV Vis spectrophotometry cuvette, if yes could you please suggest a possible method?
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Patrick Van Dijck Author Answered Oct 15, 2019

VIB Department of Molecular Microbiology, KU Leuven Laboratory of Molecular Cell Biology, Belgium

I think this can indeed be done by a proportional scale-up. If you want to use less coloring reagents or less protein extract, that might also be possible, as long as you do the same for your calibration curve and calculate everything again. Good luck!
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